A METHOD AND APPARATUS FOR THE DETECTION OF …

1. A method for detecting the presence of mycotoxins in cereals, the method comprising: capturing at least one diffuse-light absorption spectrum of a collection of cereal grains; capturing at least one diffuse-light absorption spectrum of at least one individual cereal grain from the collection of cereal grains; and classifying the level of mycotoxin contamination in at least one …

Simultaneous determination of pesticides, mycotoxins, and ...

A 2D LC-MS/MS method for the simultaneous determination of 350 pesticides, 16 mycotoxins as well as the growth regulators Chlormequat and Mepiquat was developed. The method is applicable to cereals and products thereof. Attention should be paid to the simultaneous analysis of the cereal-relevant myc …

Determination of Regulated and Emerging Mycotoxins in ...

Determination of Regulated and Emerging Mycotoxins in Cereals, Nuts, Figs, and Animal Feeds Using Pass-Through SPE and UPLC-MS/MS Nicola Dreolin, Sara Stead, Simon Hird, Timothy Jenkins Waters Corporation Abstract Mycotoxins can occur naturally in a variety of food products via pre- and post-harvest contamination mechanisms and are regulated ...

Analytical methods for determination of mycotoxins: An ...

Mycotoxins are a problematic and toxic group of small organic molecules that are produced as secondary metabolites by several fungal species that colonise crops. They lead to contamination at both the field and postharvest stages of food production with a considerable range of foodstuffs affected, from coffee and cereals, to dried fruit and spices.

Determination of mycotoxins in cereals by liquid ...

A total of 77 cereal samples (77%) contaminated with at least one of these mycotoxins. Occurrence of mycotoxins in commercial cereal samples were 70%, 40%, 25%, 36%, 19%, 13%, 16, and 16% for aflatoxins, OTA, ZEA, DON, FB 1, FB 2, T2 and HT2-toxin, respectively. The results demonstrated that the procedure was suitable for the determination of ...

Cereals and cereal products - Determination of ... - FAO

Cereals and cereal products - Determination of moisture content (Routine method) Contents - Previous - Next. 1. SCOPE AND FIELD OF APPLICATION. This International Standard specifies a routine method for the determination of the moisture content of cereals and cereal products,

Determination of 17 Mycotoxins in Cereals and Cereal …

Determination of Mycotoxins in Cereals and Cereal ased ood Usin Liquid Chromatoraphy–Triple Quadrupole Mass Spectrometry atharina von argen, Ebru Sariaya, and Michal odula Thermo Fisher Scientic, Special Solutions Center Europe, reieich, ermany Application Note 646 Key Words Commission Regulation 1881/2006/EC, mycotoxin detection

Mycotoxins (Sampling - Sample Preparation - Methods ...

Mycotoxins (Sampling - Sample Preparation - Methods) focused on Spices, Cereals, and European Union Legislation Micotoxinas (Muestreo –Preparación de la muestra –métodos) enfocado a especias, cereales y la legislación Unión Europea

Determination of 17 Mycotoxins in Cereals and Cereal …

Mycotoxins are potentially toxic secondary metabolites produced by different species of fungi. The main relevant species occurring on cereals are Penicillium sp., Fusarium sp. and Aspergillus sp. The fungal contamination can either occur on the field or during storage. For cereals and baby food, the European Union (EU) legislation currently sets maximum levels (ML) for key …

Determination of Regulated and Emerging Mycotoxins in ...

Mycotoxins can occur naturally in a variety of food products via pre- and post-harvest contamination mechanisms and are regulated worldwide. Herein we describe a multi-toxin method for 31 regulated and emerging mycotoxins using a quick and simple pass-through SPE clean-up prior to LC-MS/MS analysis for cereal-based products, ground- and tree-nuts, dried figs, and …

Evaluation of QuEChERS for mycotoxin screening

QuEChERS extraction with S and accurate mass for the analysis of T-2 and HT-2 in cereals [1]. Even in the absence of known mycotoxins, they might still occur in conjugated form and be undetected by targeted detection techniques. Soluble conjugated mycotoxins have been nominated as "masked" or "modified" mycotoxins.

Determination of Mycotoxins in Cereals by LC/MS/MS with ...

Determination of Mycotoxins in Cereals by LC/MS/MS with Online SPE. Introduction. Mycotoxins are toxic secondary metabolites produced by fungi, and are capable of causing disease and death in both humans and animals. Sample preparation is a critical step in the successful analysis of mycotoxins in food matrices. The "dilute and shoot" approach ...

Cereals and cereal products — Determination of …

Cereals and cereal products — Determination of moisture content — Reference method 1 Scope This International Standard specifies a routine reference method for the determination of the moisture content of cereals and cereal products. This International Standard applies to: wheat, rice (paddy, husked and milled), barley, millet (Panicum

Determination of Mycotoxins in Peanuts With Enhanced ...

Mycotoxins are toxic secondary metabolites produced by various mold species growing on many agricultural commodities and processed food, either in the field or during storage [3]. Many organizations regulate the levels of mycotoxins in crops such as cereal, corn, milk, and edible oils [4]. The MRLs for aflatoxin B1 in peanut are 8.0 µg/kg,

Simultaneous determination of trichothecene mycotoxins …

Method validation. The new LC-MS/MS analytical method for the determination of trichothecene mycotoxins in cereal was validated to determine the limits of detection (LOD), limits of quantification (LOQ), linearity, accuracy, and precision according to the ICH/2005/Q2/R1 guidelines (ICH, 2005).To evaluate the linearity of the matrix-matched calibration curves, a …

DETECTION METHODS FOR MYCOTOXINS IN CEREAL …

the simultaneous determination of T-2 and HT-2 after IAC clean-up in natu-rally contaminated cereal grains (wheat, maize, oats and barley). Several HPLC methods for identifying various mycotoxins in a number of cereals and cereal--based products have been validated by collaborative studies, and their perfor-

Simultaneous Determination of Multi-Mycotoxins in Cereal ...

An improved analytical method compared with conventional ones was developed for simultaneous determination of 13 mycotoxins (deoxynivalenol, nivalenol, 3-acetylnivalenol, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, fumonisin B1, fumonisin B2, T-2, HT-2, zearalenone, and ochratoxin A) in cereal grains by liquid chromatography-tandem mass spectrometry …

Simultaneous determination of 16 mycotoxins in cereals ...

Simultaneous determination of 16 mycotoxins in cereals using stable isotope labeled internal standards and e—Method by UHPLC-MS/MS Introduction Mycotoxins are toxic secondary metabolites of fungi, which can occur in various cereals, including corn, wheat, and barley. Due to the complexity of food

MANUAL OF METHODS OF ANALYSIS OF FOODS …

1.1 Safety Requirements for handling mycotoxins 5 1.2 Preparation of Sample (a) Lot Sample 6 (b) Laboratory Sample 6 1.3 Preparation of Standards for Aflatoxin 6 1.4 Preparation and storage of Working Standards 7 2.0 Determination of Aflatoxins – CB Method 7 3.0 Determination of Aflatoxins – BF Method 10

Determination of mycotoxins in cereals by liquid ...

The mean recoveries of mycotoxins in spiked cereals ranged from 76.8% to 108.4%. Limits of detection (LOD) and quantification (LOQ) ranged 0.01–20 and 0.02–40 ng/g, respectively. The developed method has been applied for the determination of mycotoxins in 100 cereal samples collected from Malaysian markets. A total of 77 cereal samples (77% ...

A validated UPLC-MS/MS multi-mycotoxin method for …

nuts and cereals ranged 0.08–30.0 and 0.25–99.0 μg/kg, respectively. Validated method was used for monitoring mycotoxins in 120 samples. A total of 97 samples (81%) were contaminated with at least one of these mycotoxins, but only the 3% of the samples exceeded the maximum levels of the EU legislation. ARTICLE HISTORY Received 31 August 2015

Simultaneous Determination of Multi-Mycotoxins in Cereal ...

An improved analytical method compared with conventional ones was developed for simultaneous determination of 13 mycotoxins (deoxynivalenol, nivalenol, 3-acetylnivalenol, aflatoxin B 1, aflatoxin B 2, aflatoxin G 1, aflatoxin G 2, fumonisin B 1, fumonisin B 2, T-2, HT-2, zearalenone, and ochratoxin A) in cereal grains by liquid chromatography-tandem mass …

Rapid and sensitive detection of mycotoxins by advanced ...

For accurate and rapid determination of these mycotoxins in unprocessed cereals and cereal-based products, sensitive, analytical methods are highly relevant to the toxicological implications to animals and humans and highly desirable in order to measure risk of exposure, further to confirm regulatory levels fixed by the United States, European ...

Simultaneous determination of major type A and B ...

A reliable and sensitive liquid chromatography-tandem mass spectrometric method was developed for the simultaneous quantitative determination in cereals of the Fusarium mycotoxins HT-2 toxin, T-2 toxin, deoxynivalenol, nivalenol and zearalenone, as well as the modified metabolites 3-acetyl-deoxynivalenol, α-zearalenol, β-zearalenol, deoxynivalenol-3 …